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anti-cxcr1 (host: rabbit, catalog#: pa5-33452)  (Thermo Fisher)
90
Thermo Fisher anti-cxcr1 (host: rabbit, catalog#: pa5-33452)
YFP fluorescence and luminescence were read as described in the Materials and Methods section. Net BRET (528/460 nm) was plotted against YFP fluorescence/luminescence (YFP/Lum). (A) HEK293T cells were transfected with AVPR1A-Rluc plus each CR-YFP in triplicate. Net BRET signals are the mean ± SD. Cells transfected with AVPR1A-Rluc and mGlu 1 R-YFP at various energy acceptor:donor ratios served as nonspecific controls; nonspecific BRET signals were analyzed by linear regression analysis. The black line shows the regression line, and the dashed lines indicate 99% prediction bands. The gray area indicates the expected distribution of nonspecific BRET signals. BRET signals above the 99% prediction band for nonspecific interactions were considered positive signals for interactions between CRs (colored symbols) and AVPR1A. The graph represents one of three screening experiments. The number of positive BRET signals in three independent screening experiments (0–3/3) is shown for each CR. (B, C, D, E, F, G, H, I, J, K) HEK293T cells were transfected with a fixed amount of AVPR1A-Rluc and with increasing amounts of CR-YFP in duplicate. Figures show saturation BRET signals representative of n = 3 independent experiments per receptor pair. Saturation BRET between AVPR1A and CCR1 (B), CCR2 (C), CCR4 (D), CCR8 (E), CXCR2 (F), CXCR3 (G), CXCR4 (H), CXCR5 (I), <t>CXCR1</t> (J), and mGlu 1 R-YFP (K).
Anti Cxcr1 (Host: Rabbit, Catalog#: Pa5 33452), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hoechst 33452  (Millipore)
90
Millipore hoechst 33452
YFP fluorescence and luminescence were read as described in the Materials and Methods section. Net BRET (528/460 nm) was plotted against YFP fluorescence/luminescence (YFP/Lum). (A) HEK293T cells were transfected with AVPR1A-Rluc plus each CR-YFP in triplicate. Net BRET signals are the mean ± SD. Cells transfected with AVPR1A-Rluc and mGlu 1 R-YFP at various energy acceptor:donor ratios served as nonspecific controls; nonspecific BRET signals were analyzed by linear regression analysis. The black line shows the regression line, and the dashed lines indicate 99% prediction bands. The gray area indicates the expected distribution of nonspecific BRET signals. BRET signals above the 99% prediction band for nonspecific interactions were considered positive signals for interactions between CRs (colored symbols) and AVPR1A. The graph represents one of three screening experiments. The number of positive BRET signals in three independent screening experiments (0–3/3) is shown for each CR. (B, C, D, E, F, G, H, I, J, K) HEK293T cells were transfected with a fixed amount of AVPR1A-Rluc and with increasing amounts of CR-YFP in duplicate. Figures show saturation BRET signals representative of n = 3 independent experiments per receptor pair. Saturation BRET between AVPR1A and CCR1 (B), CCR2 (C), CCR4 (D), CCR8 (E), CXCR2 (F), CXCR3 (G), CXCR4 (H), CXCR5 (I), <t>CXCR1</t> (J), and mGlu 1 R-YFP (K).
Hoechst 33452, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hoechst 33452 - by Bioz Stars, 2026-02
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hoechst 33452  (Dojindo Labs)
90
Dojindo Labs hoechst 33452
YFP fluorescence and luminescence were read as described in the Materials and Methods section. Net BRET (528/460 nm) was plotted against YFP fluorescence/luminescence (YFP/Lum). (A) HEK293T cells were transfected with AVPR1A-Rluc plus each CR-YFP in triplicate. Net BRET signals are the mean ± SD. Cells transfected with AVPR1A-Rluc and mGlu 1 R-YFP at various energy acceptor:donor ratios served as nonspecific controls; nonspecific BRET signals were analyzed by linear regression analysis. The black line shows the regression line, and the dashed lines indicate 99% prediction bands. The gray area indicates the expected distribution of nonspecific BRET signals. BRET signals above the 99% prediction band for nonspecific interactions were considered positive signals for interactions between CRs (colored symbols) and AVPR1A. The graph represents one of three screening experiments. The number of positive BRET signals in three independent screening experiments (0–3/3) is shown for each CR. (B, C, D, E, F, G, H, I, J, K) HEK293T cells were transfected with a fixed amount of AVPR1A-Rluc and with increasing amounts of CR-YFP in duplicate. Figures show saturation BRET signals representative of n = 3 independent experiments per receptor pair. Saturation BRET between AVPR1A and CCR1 (B), CCR2 (C), CCR4 (D), CCR8 (E), CXCR2 (F), CXCR3 (G), CXCR4 (H), CXCR5 (I), <t>CXCR1</t> (J), and mGlu 1 R-YFP (K).
Hoechst 33452, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
hoechst 33452 - by Bioz Stars, 2026-02
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hoescht 33452  (Thermo Fisher)
90
Thermo Fisher hoescht 33452
YFP fluorescence and luminescence were read as described in the Materials and Methods section. Net BRET (528/460 nm) was plotted against YFP fluorescence/luminescence (YFP/Lum). (A) HEK293T cells were transfected with AVPR1A-Rluc plus each CR-YFP in triplicate. Net BRET signals are the mean ± SD. Cells transfected with AVPR1A-Rluc and mGlu 1 R-YFP at various energy acceptor:donor ratios served as nonspecific controls; nonspecific BRET signals were analyzed by linear regression analysis. The black line shows the regression line, and the dashed lines indicate 99% prediction bands. The gray area indicates the expected distribution of nonspecific BRET signals. BRET signals above the 99% prediction band for nonspecific interactions were considered positive signals for interactions between CRs (colored symbols) and AVPR1A. The graph represents one of three screening experiments. The number of positive BRET signals in three independent screening experiments (0–3/3) is shown for each CR. (B, C, D, E, F, G, H, I, J, K) HEK293T cells were transfected with a fixed amount of AVPR1A-Rluc and with increasing amounts of CR-YFP in duplicate. Figures show saturation BRET signals representative of n = 3 independent experiments per receptor pair. Saturation BRET between AVPR1A and CCR1 (B), CCR2 (C), CCR4 (D), CCR8 (E), CXCR2 (F), CXCR3 (G), CXCR4 (H), CXCR5 (I), <t>CXCR1</t> (J), and mGlu 1 R-YFP (K).
Hoescht 33452, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hoescht 33452/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
hoescht 33452 - by Bioz Stars, 2026-02
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hoechst 33452  (Thermo Fisher)
90
Thermo Fisher hoechst 33452
YFP fluorescence and luminescence were read as described in the Materials and Methods section. Net BRET (528/460 nm) was plotted against YFP fluorescence/luminescence (YFP/Lum). (A) HEK293T cells were transfected with AVPR1A-Rluc plus each CR-YFP in triplicate. Net BRET signals are the mean ± SD. Cells transfected with AVPR1A-Rluc and mGlu 1 R-YFP at various energy acceptor:donor ratios served as nonspecific controls; nonspecific BRET signals were analyzed by linear regression analysis. The black line shows the regression line, and the dashed lines indicate 99% prediction bands. The gray area indicates the expected distribution of nonspecific BRET signals. BRET signals above the 99% prediction band for nonspecific interactions were considered positive signals for interactions between CRs (colored symbols) and AVPR1A. The graph represents one of three screening experiments. The number of positive BRET signals in three independent screening experiments (0–3/3) is shown for each CR. (B, C, D, E, F, G, H, I, J, K) HEK293T cells were transfected with a fixed amount of AVPR1A-Rluc and with increasing amounts of CR-YFP in duplicate. Figures show saturation BRET signals representative of n = 3 independent experiments per receptor pair. Saturation BRET between AVPR1A and CCR1 (B), CCR2 (C), CCR4 (D), CCR8 (E), CXCR2 (F), CXCR3 (G), CXCR4 (H), CXCR5 (I), <t>CXCR1</t> (J), and mGlu 1 R-YFP (K).
Hoechst 33452, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hoechst 33452/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
hoechst 33452 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


YFP fluorescence and luminescence were read as described in the Materials and Methods section. Net BRET (528/460 nm) was plotted against YFP fluorescence/luminescence (YFP/Lum). (A) HEK293T cells were transfected with AVPR1A-Rluc plus each CR-YFP in triplicate. Net BRET signals are the mean ± SD. Cells transfected with AVPR1A-Rluc and mGlu 1 R-YFP at various energy acceptor:donor ratios served as nonspecific controls; nonspecific BRET signals were analyzed by linear regression analysis. The black line shows the regression line, and the dashed lines indicate 99% prediction bands. The gray area indicates the expected distribution of nonspecific BRET signals. BRET signals above the 99% prediction band for nonspecific interactions were considered positive signals for interactions between CRs (colored symbols) and AVPR1A. The graph represents one of three screening experiments. The number of positive BRET signals in three independent screening experiments (0–3/3) is shown for each CR. (B, C, D, E, F, G, H, I, J, K) HEK293T cells were transfected with a fixed amount of AVPR1A-Rluc and with increasing amounts of CR-YFP in duplicate. Figures show saturation BRET signals representative of n = 3 independent experiments per receptor pair. Saturation BRET between AVPR1A and CCR1 (B), CCR2 (C), CCR4 (D), CCR8 (E), CXCR2 (F), CXCR3 (G), CXCR4 (H), CXCR5 (I), CXCR1 (J), and mGlu 1 R-YFP (K).

Journal: Life Science Alliance

Article Title: Chemokine receptor hetero-oligomers regulate monocyte chemotaxis

doi: 10.26508/lsa.202402657

Figure Lengend Snippet: YFP fluorescence and luminescence were read as described in the Materials and Methods section. Net BRET (528/460 nm) was plotted against YFP fluorescence/luminescence (YFP/Lum). (A) HEK293T cells were transfected with AVPR1A-Rluc plus each CR-YFP in triplicate. Net BRET signals are the mean ± SD. Cells transfected with AVPR1A-Rluc and mGlu 1 R-YFP at various energy acceptor:donor ratios served as nonspecific controls; nonspecific BRET signals were analyzed by linear regression analysis. The black line shows the regression line, and the dashed lines indicate 99% prediction bands. The gray area indicates the expected distribution of nonspecific BRET signals. BRET signals above the 99% prediction band for nonspecific interactions were considered positive signals for interactions between CRs (colored symbols) and AVPR1A. The graph represents one of three screening experiments. The number of positive BRET signals in three independent screening experiments (0–3/3) is shown for each CR. (B, C, D, E, F, G, H, I, J, K) HEK293T cells were transfected with a fixed amount of AVPR1A-Rluc and with increasing amounts of CR-YFP in duplicate. Figures show saturation BRET signals representative of n = 3 independent experiments per receptor pair. Saturation BRET between AVPR1A and CCR1 (B), CCR2 (C), CCR4 (D), CCR8 (E), CXCR2 (F), CXCR3 (G), CXCR4 (H), CXCR5 (I), CXCR1 (J), and mGlu 1 R-YFP (K).

Article Snippet: The antibodies anti-α 1B -AR (host: rabbit; catalog#: ab169523), anti-α 1D -AR (host: rabbit; catalog#: ab84402), and anti-CXCR4 (host: goat, ab1670) were obtained from Abcam; anti-AVPR1A (host: rabbit; catalog#: bs-11598R) was from Bioss; anti-AVPR1A (host: mouse, catalog#: LS-C126889) and anti-CCR8 (host: goat; catalog#: LS-C187704) were from LifeSpan Biosciences; anti-CXCR1 (host: rabbit, catalog#: PA5-33452) was from Invitrogen; and anti-CCR1 (host: mouse; catalog#: MAB145), anti-CCR2 (host: mouse; catalog#: MAB48607), IgG isotype control (host: rabbit, catalog#: MAB1050), IgG isotype control (host: mouse, catalog#: MAB004), and IgG isotype control (host: goat, catalog#: AB-108-C) were from R&D Systems.

Techniques: Fluorescence, Transfection

(A, C) THP-1 cells were incubated with vehicle (ctrl., top), 10 μmol/liter arginine vasopressin (aVP, center), or 10 μmol/liter conivaptan (con, bottom) for 30 min at 37°C, and the cell surface expression of individual receptors (A) and receptor–receptor proximity (C) was visualized by the proximity ligation assay (PLA). Images show merged DAPI (nuclear counterstain) and PLA signals (red, λ excitation/emission 598/634 nm) acquired from z-stack images (n = 10; thickness 0.5 μm, bottom to top) and are representative of n = 3 independent experiments. Scale bar, 10 μm. (B) Quantification of PLA signals for AVPR1A, CCR1, CCR2, CCR8, CXCR1, and CXCR4 from n = 3 experiments. Data are the mean ± SE. * P < 0.05 versus ctrl. (D) Quantification of PLA signals for receptor–receptor proximity between AVPR1A and CCR1, CCR2, CCR8, or CXCR4 from n = 3 experiments. Data are the mean ± SE. * P < 0.05 versus ctrl.

Journal: Life Science Alliance

Article Title: Chemokine receptor hetero-oligomers regulate monocyte chemotaxis

doi: 10.26508/lsa.202402657

Figure Lengend Snippet: (A, C) THP-1 cells were incubated with vehicle (ctrl., top), 10 μmol/liter arginine vasopressin (aVP, center), or 10 μmol/liter conivaptan (con, bottom) for 30 min at 37°C, and the cell surface expression of individual receptors (A) and receptor–receptor proximity (C) was visualized by the proximity ligation assay (PLA). Images show merged DAPI (nuclear counterstain) and PLA signals (red, λ excitation/emission 598/634 nm) acquired from z-stack images (n = 10; thickness 0.5 μm, bottom to top) and are representative of n = 3 independent experiments. Scale bar, 10 μm. (B) Quantification of PLA signals for AVPR1A, CCR1, CCR2, CCR8, CXCR1, and CXCR4 from n = 3 experiments. Data are the mean ± SE. * P < 0.05 versus ctrl. (D) Quantification of PLA signals for receptor–receptor proximity between AVPR1A and CCR1, CCR2, CCR8, or CXCR4 from n = 3 experiments. Data are the mean ± SE. * P < 0.05 versus ctrl.

Article Snippet: The antibodies anti-α 1B -AR (host: rabbit; catalog#: ab169523), anti-α 1D -AR (host: rabbit; catalog#: ab84402), and anti-CXCR4 (host: goat, ab1670) were obtained from Abcam; anti-AVPR1A (host: rabbit; catalog#: bs-11598R) was from Bioss; anti-AVPR1A (host: mouse, catalog#: LS-C126889) and anti-CCR8 (host: goat; catalog#: LS-C187704) were from LifeSpan Biosciences; anti-CXCR1 (host: rabbit, catalog#: PA5-33452) was from Invitrogen; and anti-CCR1 (host: mouse; catalog#: MAB145), anti-CCR2 (host: mouse; catalog#: MAB48607), IgG isotype control (host: rabbit, catalog#: MAB1050), IgG isotype control (host: mouse, catalog#: MAB004), and IgG isotype control (host: goat, catalog#: AB-108-C) were from R&D Systems.

Techniques: Incubation, Expressing, Proximity Ligation Assay

(A, B, C, D, E, F, G) THP-1 cells were incubated with NT or AVPR1A siRNA. (A) Representative images for the detection of AVPR1A and proximity between AVPR1A and CCR1, CCR2, CCR8, CXCR4, and CXCR1 by the proximity ligation assay (PLA). Images show merged DAPI/PLA signals and are representative of n = 3 independent experiments. Scale bar, 10 μm. (B, C, D, E, F, G) Quantification of the number of PLA signals per cell for AVPR1A and receptor–receptor proximities in THP-1 cells after incubation with NT siRNA (ctrl., white bars) or AVPR1A siRNA (gray bars) (n = 3). Data are the mean ± SE. * P < 0.05 versus cells treated with NT siRNA. (H, I, J, K) THP-1 cells were incubated with NT or AVPR1A siRNA as in (A, B, C, D, E, F, G). Cells were then exposed to various concentrations of aVP, and chemotaxis toward CCL23 (0.1 nmol/liter, (H)), CCL2 (10 nmol/liter, (I)), CXCL12 (100 nmol/liter, (J)), and CXCL8 (10 nmol/liter, (K)) was tested. CI, chemotactic index (mean ± SE, n = 3/condition). * P < 0.05 versus cells incubated with NT siRNA (two-way ANOVA with Dunnett’s multiple comparisons test).

Journal: Life Science Alliance

Article Title: Chemokine receptor hetero-oligomers regulate monocyte chemotaxis

doi: 10.26508/lsa.202402657

Figure Lengend Snippet: (A, B, C, D, E, F, G) THP-1 cells were incubated with NT or AVPR1A siRNA. (A) Representative images for the detection of AVPR1A and proximity between AVPR1A and CCR1, CCR2, CCR8, CXCR4, and CXCR1 by the proximity ligation assay (PLA). Images show merged DAPI/PLA signals and are representative of n = 3 independent experiments. Scale bar, 10 μm. (B, C, D, E, F, G) Quantification of the number of PLA signals per cell for AVPR1A and receptor–receptor proximities in THP-1 cells after incubation with NT siRNA (ctrl., white bars) or AVPR1A siRNA (gray bars) (n = 3). Data are the mean ± SE. * P < 0.05 versus cells treated with NT siRNA. (H, I, J, K) THP-1 cells were incubated with NT or AVPR1A siRNA as in (A, B, C, D, E, F, G). Cells were then exposed to various concentrations of aVP, and chemotaxis toward CCL23 (0.1 nmol/liter, (H)), CCL2 (10 nmol/liter, (I)), CXCL12 (100 nmol/liter, (J)), and CXCL8 (10 nmol/liter, (K)) was tested. CI, chemotactic index (mean ± SE, n = 3/condition). * P < 0.05 versus cells incubated with NT siRNA (two-way ANOVA with Dunnett’s multiple comparisons test).

Article Snippet: The antibodies anti-α 1B -AR (host: rabbit; catalog#: ab169523), anti-α 1D -AR (host: rabbit; catalog#: ab84402), and anti-CXCR4 (host: goat, ab1670) were obtained from Abcam; anti-AVPR1A (host: rabbit; catalog#: bs-11598R) was from Bioss; anti-AVPR1A (host: mouse, catalog#: LS-C126889) and anti-CCR8 (host: goat; catalog#: LS-C187704) were from LifeSpan Biosciences; anti-CXCR1 (host: rabbit, catalog#: PA5-33452) was from Invitrogen; and anti-CCR1 (host: mouse; catalog#: MAB145), anti-CCR2 (host: mouse; catalog#: MAB48607), IgG isotype control (host: rabbit, catalog#: MAB1050), IgG isotype control (host: mouse, catalog#: MAB004), and IgG isotype control (host: goat, catalog#: AB-108-C) were from R&D Systems.

Techniques: Incubation, Proximity Ligation Assay, Chemotaxis Assay